Perturbing gene expression with baculovirus
نویسندگان
چکیده
منابع مشابه
Baculovirus and insect cell gene expression: review of baculovirus biotechnology.
The BEVS continues to evolve as a powerful, flexible tool for molecular biology, protein function, and biomedical research. Future developments offer the promise of replacement of hazardous chemical insecticides with environmentally safe biopesticides, construction of baculovirus vectors which encode genes for specific post-translational modifications, and establishment of efficient, stably tra...
متن کاملPerturbing Chromatin Structure to Understand Mechanisms of Gene Expression.
The study of nuclear structure falls between the fields of cell biology and molecular biology and draws on techniques from both fields. In recent years, many exciting advances have been made in these areas, including single-molecule and superresolution imaging and the development of chromosome conformation capture (3C)-based technologies, which have brought the advent of genome-wide analysis of...
متن کاملNucleic Acid–based Strategies for Perturbing Gene Expression—a Primer
AS WE APPROACH the new millennium, a quick glance backwards reveals that truly astounding progress has been made in the identification of genes responsible for cell growth, development, and neoplastic transformation. With this knowledge has come a natural desire to ‘‘translate’’ this information into new therapeutic strategies for many of the common maladies that afflict humankind. These includ...
متن کاملBaculovirus gp64 gene expression: negative regulation by a minicistron.
Small upstream open reading frames (ORFs) or minicistrons located in the 5' leader of eukaryotic mRNAs have been shown to play a role in translational regulation of some eukaryotic genes, particularly mammalian proto-oncogenes. A survey of the baculovirus Autographa californica multicapsid nuclear polyhedrosis virus genome suggests that at least 10 transcripts from late genes contain potential ...
متن کاملBaculovirus expression vectors with single strand capability.
Recombinant baculoviruses offer a useful way of expressing eucaryotic genes, often in high yield (1). Current procedures for the generation of a recombinant virus make use of a transfer vector that, via recombination in vivo, place the gene of interest under control of the powerful polyhedrin promotor. At present the expression of mutant forms of the cloned gene (to study structure and function...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genome Biology
سال: 2000
ISSN: 1474-760X
DOI: 10.1186/gb-2000-1-1-reports012